8JIT

Cryo-EM structure of the GLP-1R/GCGR dual agonist MEDI0382-bound human GCGR-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	P	MEDI0382	polymer	29	3307.5	1		synthetic construct
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
5	N	Nanobody 35	polymer	140	15343.0	1		Escherichia coli
6	R	Glucagon receptor	polymer	405	46627.3	1	UniProt (P47871) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	GL-R
7	P	N-hexadecanoyl-L-glutamic acid	non-polymer		385.5	1	Chemie (D6M)

Sequence modifications

A: -5 - 378 (UniProt: P63092)

PDB	External Database	Details
Asn 48	Ser 54	conflict
Ala 210	Gly 226	conflict
Ala 252	Glu 268	conflict
Lys 255	Asn 271	conflict
Asp 258	Lys 274	conflict
Lys 264	Arg 280	conflict
Asp 268	Thr 284	conflict
Thr 269	Ile 285	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	156606.7
Non-Polymers*	Number of molecules	1
	Total formula weight	385.5
All*	Total formula weight	156992.2

*Water molecules are not included.