8JI0

Cryo-EM structure of the TcsH-CROP in complex with TMPRSS2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Transmembrane protease serine 2	polymer	424	46713.0	1	UniProt (O15393) Pfam (PF15494) Pfam (PF00089)	Homo sapiens (human)	Serine protease 10
2	B (A)	Maltose/maltodextrin-binding periplasmic protein,Hemorrhagic toxin	polymer	786	87735.6	1	UniProt (P0AEX9) UniProt (M9ZTT7) Pfam (PF01547)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,TcsH

Sequence modifications

B: 106 - 492 (UniProt: O15393)

PDB	External Database	Details
Met 69	-	initiating methionine
Gly 70	-	expression tag
Ile 71	-	expression tag
Leu 72	-	expression tag
Pro 73	-	expression tag
Ser 74	-	expression tag
Pro 75	-	expression tag
Gly 76	-	expression tag
Met 77	-	expression tag
Pro 78	-	expression tag
Ala 79	-	expression tag
Leu 80	-	expression tag
Leu 81	-	expression tag
Ser 82	-	expression tag
Leu 83	-	expression tag
Val 84	-	expression tag
Ser 85	-	expression tag
Leu 86	-	expression tag
Leu 87	-	expression tag
Ser 88	-	expression tag
Val 89	-	expression tag
Leu 90	-	expression tag
Leu 91	-	expression tag
Met 92	-	expression tag
Gly 93	-	expression tag
Cys 94	-	expression tag
Val 95	-	expression tag
Ala 96	-	expression tag
Glu 97	-	expression tag
Thr 98	-	expression tag
Gly 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
Gln 255	Arg 255	conflict

A: 1856 - 2221 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1833	-	initiating methionine
Ala 1834	-	expression tag
Ser 1835	-	expression tag
Met 1836	-	expression tag
Thr 1837	-	expression tag
Gly 1838	-	expression tag
Gly 1839	-	expression tag
Gln 1840	-	expression tag
Gln 1841	-	expression tag
Met 1842	-	expression tag
Gly 1843	-	expression tag
Arg 1844	-	expression tag
Gly 1845	-	expression tag
Ser 1846	-	expression tag
His 1847	-	expression tag
His 1848	-	expression tag
His 1849	-	expression tag
His 1850	-	expression tag
His 1851	-	expression tag
His 1852	-	expression tag
His 1853	-	expression tag
His 1854	-	expression tag
Met 1855	-	expression tag
Gly 2222	-	linker
Ser 2223	-	linker
Ser 2224	-	linker
Ser 2225	-	linker
Leu 2226	-	linker
Glu 2227	-	linker
Val 2228	-	linker
Leu 2229	-	linker
Phe 2230	-	linker
Gln 2231	-	linker
Gly 2232	-	linker
Pro 2233	-	linker
Glu 2234	-	linker
Phe 2235	-	linker

A: 2236 - 2618 (UniProt: M9ZTT7)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	134448.6
All*	Total formula weight	134448.6

*Water molecules are not included.