8JF7
Triheteromeric NMDA receptor GluN1-GluN2A-GluN3A in complex with glycine, glutamate, a GluN1-specific Fab,and a GluN2A-specific Fab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Glutamate receptor ionotropic, NMDA 1 | polymer | 847 | 95372.2 | 2 | UniProt (P35439) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) | Rattus norvegicus (Norway rat) | GluN1,Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1,NMD-R1 |
| 2 | B (B) | Glutamate receptor ionotropic, NMDA 2A,Enhanced green fluorescent protein | polymer | 1116 | 125326.0 | 1 | UniProt (Q00959) UniProt (A0A7G8ZY66) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) Pfam (PF01353) UniProt (by SIFTS) (P42212) | Rattus norvegicus (Norway rat) | GluN2A,Glutamate [NMDA] receptor subunit epsilon-1,N-methyl D-aspartate receptor subtype 2A,NMDAR2A,NR2A |
| 3 | D (D) | Glutamate receptor ionotropic, NMDA 3A,Enhanced green fluorescent protein | polymer | 1228 | 137362.8 | 1 | UniProt (Q9R1M7) UniProt (A0A7G8ZY66) Pfam (PF00060) Pfam (PF01353) Pfam (PF01094) Pfam (PF10613) UniProt (by SIFTS) (P42212) | Rattus norvegicus (Norway rat) | GluN3A,Glutamate receptor chi-1,N-methyl-D-aspartate receptor,N-methyl-D-aspartate receptor subtype 3A,NMDAR3A,NR3A,NMDAR-L,NMDAR-L1 |
| 4 | E, G (E, G) | GluN1-specific antibody fragment light chain | polymer | 237 | 25739.4 | 2 | Mus musculus (house mouse) | ||
| 5 | F, H (F, H) | GluN1-specific antibody fragment heavy chain | polymer | 217 | 23432.1 | 2 | Mus musculus (house mouse) | ||
| 6 | I (K) | GluN2A-specific antibody fragment light chain | polymer | 212 | 23234.7 | 1 | Mus musculus (house mouse) | ||
| 7 | J (J) | GluN2A-specific antibody fragment heavy chain | polymer | 227 | 24936.9 | 1 | Mus musculus (house mouse) |
Sequence modifications
B: 1 - 842 (UniProt: Q00959)
B: 867 - 1108 (UniProt: A0A7G8ZY66)
D: 1 - 965 (UniProt: Q9R1M7)
D: 979 - 1220 (UniProt: A0A7G8ZY66)
| PDB | External Database | Details |
|---|---|---|
| Lys 843 | - | linker |
| Ser 844 | - | linker |
| Arg 845 | - | linker |
| Ala 846 | - | linker |
| Glu 847 | - | linker |
| Ala 848 | - | linker |
| Lys 849 | - | linker |
| Arg 850 | - | linker |
| Met 851 | - | linker |
| Lys 852 | - | linker |
| Gly 853 | - | linker |
| Leu 854 | - | linker |
| Glu 855 | - | linker |
| Val 856 | - | linker |
| Leu 857 | - | linker |
| Phe 858 | - | linker |
| Gln 859 | - | linker |
| Gly 860 | - | linker |
| Pro 861 | - | linker |
| Ala 862 | - | linker |
| Ala 863 | - | linker |
| Ala 864 | - | linker |
| Ala 865 | - | linker |
| Val 866 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Lys 1071 | Ala 207 | conflict |
| His 1109 | - | expression tag |
| His 1110 | - | expression tag |
| His 1111 | - | expression tag |
| His 1112 | - | expression tag |
| His 1113 | - | expression tag |
| His 1114 | - | expression tag |
| His 1115 | - | expression tag |
| His 1116 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 927 | Gly 927 | conflict |
| Leu 966 | - | linker |
| Glu 967 | - | linker |
| Val 968 | - | linker |
| Leu 969 | - | linker |
| Phe 970 | - | linker |
| Gln 971 | - | linker |
| Gly 972 | - | linker |
| Pro 973 | - | linker |
| Ala 974 | - | linker |
| Ala 975 | - | linker |
| Ala 976 | - | linker |
| Ala 977 | - | linker |
| Val 978 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Lys 1183 | Ala 207 | conflict |
| Trp 1221 | - | expression tag |
| Ser 1222 | - | expression tag |
| His 1223 | - | expression tag |
| Pro 1224 | - | expression tag |
| Gln 1225 | - | expression tag |
| Phe 1226 | - | expression tag |
| Glu 1227 | - | expression tag |
| Lys 1228 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 599947.8 | |
| All* | Total formula weight | 599947.8 |
