8JE1

An asymmetry dimer of the Cul2-Rbx1-EloBC-FEM1B ubiquitin ligase complexed with BEX2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F	Cullin-2	polymer	751	87927.8	2	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (human)	CUL-2
2	B, G	Elongin-B	polymer	104	11748.4	2	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
3	C, H	Elongin-C	polymer	98	11045.7	2	UniProt (Q15369) Pfam (PF03931) In PDB	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
4	D, I	Protein fem-1 homolog B	polymer	630	70688.4	2	UniProt (Q9UK73) Pfam (PF12796) In PDB	Homo sapiens (human)	FEM1b,FEM1-beta,Fem-1-like death receptor-binding protein alpha,Fem-1-like in apoptotic pathway protein alpha,F1A-alpha
5	R	E3 ubiquitin-protein ligase RBX1	polymer	108	12290.0	1	UniProt (P62877) Pfam (PF12678) In PDB	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
6	I	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, F: 1 - 745 (UniProt: Q13617)

PDB	External Database	Details
His 746	-	expression tag
His 747	-	expression tag
His 748	-	expression tag
His 749	-	expression tag
His 750	-	expression tag
His 751	-	expression tag

C, H: 16 - 112 (UniProt: Q15369)

PDB	External Database	Details
Met 15	-	initiating methionine

D, I: 1 - 627 (UniProt: Q9UK73)

PDB	External Database	Details
Gly -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	375110.6
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	375176.0

*Water molecules are not included.