Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8JE1

An asymmetry dimer of the Cul2-Rbx1-EloBC-FEM1B ubiquitin ligase complexed with BEX2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000082	biological_process	G1/S transition of mitotic cell cycle
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005829	cellular_component	cytosol
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010498	biological_process	proteasomal protein catabolic process
A	0016567	biological_process	protein ubiquitination
A	0019005	cellular_component	SCF ubiquitin ligase complex
A	0030163	biological_process	protein catabolic process
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0030891	cellular_component	VCB complex
A	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
A	0031461	cellular_component	cullin-RING ubiquitin ligase complex
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0044877	molecular_function	protein-containing complex binding
A	0097193	biological_process	intrinsic apoptotic signaling pathway
A	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A	0160072	molecular_function	ubiquitin ligase complex scaffold activity
B	0006368	biological_process	transcription elongation by RNA polymerase II
B	0030891	cellular_component	VCB complex
B	0070449	cellular_component	elongin complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0000151	cellular_component	ubiquitin ligase complex
D	0002070	biological_process	epithelial cell maturation
D	0005123	molecular_function	death receptor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006915	biological_process	apoptotic process
D	0016567	biological_process	protein ubiquitination
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0046872	molecular_function	metal ion binding
D	0051438	biological_process	regulation of ubiquitin-protein transferase activity
D	0060442	biological_process	branching involved in prostate gland morphogenesis
D	0060743	biological_process	epithelial cell maturation involved in prostate gland development
D	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
D	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
D	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
D	2000001	biological_process	regulation of DNA damage checkpoint
F	0000082	biological_process	G1/S transition of mitotic cell cycle
F	0004842	molecular_function	ubiquitin-protein transferase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005730	cellular_component	nucleolus
F	0005829	cellular_component	cytosol
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0010498	biological_process	proteasomal protein catabolic process
F	0016567	biological_process	protein ubiquitination
F	0019005	cellular_component	SCF ubiquitin ligase complex
F	0030163	biological_process	protein catabolic process
F	0030674	molecular_function	protein-macromolecule adaptor activity
F	0030891	cellular_component	VCB complex
F	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
F	0031461	cellular_component	cullin-RING ubiquitin ligase complex
F	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0044877	molecular_function	protein-containing complex binding
F	0097193	biological_process	intrinsic apoptotic signaling pathway
F	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
F	0160072	molecular_function	ubiquitin ligase complex scaffold activity
G	0006368	biological_process	transcription elongation by RNA polymerase II
G	0030891	cellular_component	VCB complex
G	0070449	cellular_component	elongin complex
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
I	0000151	cellular_component	ubiquitin ligase complex
I	0002070	biological_process	epithelial cell maturation
I	0005123	molecular_function	death receptor binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005739	cellular_component	mitochondrion
I	0005829	cellular_component	cytosol
I	0006915	biological_process	apoptotic process
I	0016567	biological_process	protein ubiquitination
I	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0046872	molecular_function	metal ion binding
I	0051438	biological_process	regulation of ubiquitin-protein transferase activity
I	0060442	biological_process	branching involved in prostate gland morphogenesis
I	0060743	biological_process	epithelial cell maturation involved in prostate gland development
I	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
I	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
I	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
I	2000001	biological_process	regulation of DNA damage checkpoint
R	0000165	biological_process	MAPK cascade
R	0000209	biological_process	protein polyubiquitination
R	0004842	molecular_function	ubiquitin-protein transferase activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006281	biological_process	DNA repair
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0006513	biological_process	protein monoubiquitination
R	0006974	biological_process	DNA damage response
R	0007283	biological_process	spermatogenesis
R	0008270	molecular_function	zinc ion binding
R	0016567	biological_process	protein ubiquitination
R	0016740	molecular_function	transferase activity
R	0019005	cellular_component	SCF ubiquitin ligase complex
R	0019788	molecular_function	NEDD8 transferase activity
R	0030163	biological_process	protein catabolic process
R	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R	0031461	cellular_component	cullin-RING ubiquitin ligase complex
R	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
R	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
R	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
R	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
R	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
R	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
R	0031625	molecular_function	ubiquitin protein ligase binding
R	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
R	0032480	biological_process	negative regulation of type I interferon production
R	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
R	0034599	biological_process	cellular response to oxidative stress
R	0034644	biological_process	cellular response to UV
R	0042110	biological_process	T cell activation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0043687	biological_process	post-translational protein modification
R	0045116	biological_process	protein neddylation
R	0045732	biological_process	positive regulation of protein catabolic process
R	0046872	molecular_function	metal ion binding
R	0060090	molecular_function	molecular adaptor activity
R	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
R	0061630	molecular_function	ubiquitin protein ligase activity
R	0061663	molecular_function	NEDD8 ligase activity
R	0062197	biological_process	cellular response to chemical stress
R	0070936	biological_process	protein K48-linked ubiquitination
R	0071230	biological_process	cellular response to amino acid stimulus
R	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
R	0097602	molecular_function	cullin family protein binding
R	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
R	1900076	biological_process	regulation of cellular response to insulin stimulus
R	1902499	biological_process	positive regulation of protein autoubiquitination
R	1902883	biological_process	negative regulation of response to oxidative stress
R	1904263	biological_process	positive regulation of TORC1 signaling

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
A	ILE718-ALA745

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	45
Details	ZN_FING: RING-type => ECO:0000255\|PROSITE-ProRule:PRU00175

Chain	Residue	Details
R	CYS53-ASN98
D	CYS186
D	HIS218
I	HIS185
I	CYS186
I	HIS218

site_id	SWS_FT_FI2
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269\|PubMed:11961546, ECO:0007744\|PDB:1LDJ, ECO:0007744\|PDB:1LDK, ECO:0007744\|PDB:1U6G, ECO:0007744\|PDB:2HYE, ECO:0007744\|PDB:3DPL, ECO:0007744\|PDB:3DQV, ECO:0007744\|PDB:3RTR, ECO:0007744\|PDB:4F52, ECO:0007744\|PDB:4P5O

Chain	Residue	Details
R	CYS42
R	CYS45
R	CYS75
R	HIS77
R	HIS80
R	CYS94
R	ASP97

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:11961546, ECO:0007744\|PDB:1LDJ, ECO:0007744\|PDB:1LDK, ECO:0007744\|PDB:1U6G, ECO:0007744\|PDB:2HYE, ECO:0007744\|PDB:3DPL, ECO:0007744\|PDB:3DQV, ECO:0007744\|PDB:3RTR, ECO:0007744\|PDB:4F52, ECO:0007744\|PDB:4P5O, ECO:0007744\|PDB:7Z8B

Chain	Residue	Details
R	CYS53
R	CYS56
R	CYS68
R	HIS82

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269\|PubMed:11961546, ECO:0007744\|PDB:1LDJ, ECO:0007744\|PDB:1LDK, ECO:0007744\|PDB:1U6G, ECO:0007744\|PDB:2HYE, ECO:0007744\|PDB:3DQV, ECO:0007744\|PDB:3RTR, ECO:0007744\|PDB:4F52, ECO:0007744\|PDB:4P5O

Chain	Residue	Details
R	CYS83

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0007744\|PubMed:20068231

Chain	Residue	Details
R	MET1

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed => ECO:0000269\|Ref.8, ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:22223895, ECO:0007744\|PubMed:22814378

Chain	Residue	Details
R	ALA2

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
R	THR9

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)