8JD5

Cryo-EM structure of Gi1-bound mGlu2-mGlu4 heterodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	Metabotropic glutamate receptor 2	polymer	870	95684.3	1	UniProt (Q14416) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003)	Homo sapiens (human)	mGluR2
2	B (4)	Metabotropic glutamate receptor 4	polymer	916	102560.8	1	UniProt (Q14833) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003)	Homo sapiens (human)	mGluR4
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (H)	scFv	polymer	257	27409.6	1		Escherichia coli
7	G (2)	1-butyl-3-chloranyl-4-(4-phenylpiperidin-1-yl)pyridin-2-one	non-polymer		344.9	1	Chemie (HZR)
8	H, O (2, 4)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
9	I, L, M, N, R (2, 4)	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)
10	J (2)	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	1	Chemie (PEF)
11	K, P (2, 4)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
12	Q (4)	5-methyl-N-(4-methylpyrimidin-2-yl)-4-(1H-pyrazol-4-yl)-1,3-thiazol-2-amine	non-polymer		272.3	1	Chemie (BQI)

Sequence modifications

2: 19 - 872 (UniProt: Q14416)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Pro 18	-	expression tag
Leu 873	-	expression tag
Glu 874	-	expression tag
Val 875	-	expression tag
Leu 876	-	expression tag
Phe 877	-	expression tag
Gln 878	-	expression tag

4: 33 - 912 (UniProt: Q14833)

PDB	External Database	Details
Asp 3	-	expression tag
Tyr 4	-	expression tag
Lys 5	-	expression tag
Asp 6	-	expression tag
Asp 7	-	expression tag
Asp 8	-	expression tag
Asp 9	-	expression tag
Gly 10	-	expression tag
Ala 11	-	expression tag
Pro 12	-	expression tag
Trp 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Pro 16	-	expression tag
Gln 17	-	expression tag
Phe 18	-	expression tag
Glu 19	-	expression tag
Lys 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
Trp 25	-	expression tag
Ser 26	-	expression tag
His 27	-	expression tag
Pro 28	-	expression tag
Gln 29	-	expression tag
Phe 30	-	expression tag
Glu 31	-	expression tag
Lys 32	-	expression tag
Leu 913	-	expression tag
Glu 914	-	expression tag
Val 915	-	expression tag
Leu 916	-	expression tag
Phe 917	-	expression tag
Gln 918	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	312674.2
Non-Polymers*	Number of molecules	12
	Total formula weight	3979.1
All*	Total formula weight	316653.3

*Water molecules are not included.