8JD4

Cryo-EM structure of G protein-free mGlu2-mGlu4 heterodimer in Acc state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	Metabotropic glutamate receptor 2,Peptidyl-prolyl cis-trans isomerase FKBP1A	polymer	993	109398.9	1	UniProt (Q14416) UniProt (P62942) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) Pfam (PF00254)	Homo sapiens (human)	mGluR2,PPIase FKBP1A,12 kDa FK506-binding protein,12 kDa FKBP,FKBP-12,Calstabin-1,FK506-binding protein 1A,FKBP-1A,Immunophilin FKBP12,Rotamase
2	B (4)	Metabotropic glutamate receptor 4,Serine/threonine-protein kinase mTOR	polymer	1013	114187.1	1	UniProt (Q14833) UniProt (A0A8V8TRG9) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) Pfam (PF08771)	Homo sapiens (human)	mGluR4
3	C, D, G (2, 4)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	E, F (2, 4)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)

Sequence modifications

2: 19 - 872 (UniProt: Q14416)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Pro 18	-	expression tag
Leu 873	-	linker
Glu 874	-	linker
Val 875	-	linker
Leu 876	-	linker
Phe 877	-	linker
Gln 878	-	linker
Gly 879	-	linker
Pro 880	-	linker

2: 881 - 987 (UniProt: P62942)

PDB	External Database	Details
Phe 988	-	expression tag
Ala 989	-	expression tag
Ala 990	-	expression tag
Ala 991	-	expression tag
His 992	-	expression tag
His 993	-	expression tag
His 994	-	expression tag
His 995	-	expression tag
His 996	-	expression tag
His 997	-	expression tag
His 998	-	expression tag
His 999	-	expression tag
His 1000	-	expression tag
His 1001	-	expression tag

4: 33 - 912 (UniProt: Q14833)

PDB	External Database	Details
Asp 3	-	expression tag
Tyr 4	-	expression tag
Lys 5	-	expression tag
Asp 6	-	expression tag
Asp 7	-	expression tag
Asp 8	-	expression tag
Asp 9	-	expression tag
Gly 10	-	expression tag
Ala 11	-	expression tag
Pro 12	-	expression tag
Trp 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Pro 16	-	expression tag
Gln 17	-	expression tag
Phe 18	-	expression tag
Glu 19	-	expression tag
Lys 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
Trp 25	-	expression tag
Ser 26	-	expression tag
His 27	-	expression tag
Pro 28	-	expression tag
Gln 29	-	expression tag
Phe 30	-	expression tag
Glu 31	-	expression tag
Lys 32	-	expression tag
Leu 913	-	linker
Glu 914	-	linker
Val 915	-	linker
Leu 916	-	linker
Phe 917	-	linker
Gln 918	-	linker
Gly 919	-	linker
Pro 920	-	linker

4: 921 - 1015 (UniProt: A0A8V8TRG9)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	223586.0
Non-Polymers*	Number of molecules	5
	Total formula weight	957.9
All*	Total formula weight	224543.9

*Water molecules are not included.