8JD3

Cryo-EM structure of Gi1-bound mGlu2-mGlu3 heterodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	Metabotropic glutamate receptor 2	polymer	870	95684.3	1	UniProt (Q14416) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003)	Homo sapiens (human)	mGluR2
2	B (3)	Metabotropic glutamate receptor 3	polymer	894	100810.3	1	UniProt (Q14832) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003)	Homo sapiens (human)	mGluR3
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (2)	1-butyl-3-chloranyl-4-(4-phenylpiperidin-1-yl)pyridin-2-one	non-polymer		344.9	1	Chemie (HZR)
7	G, I (2, 3)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
8	H, J, K (2, 3)	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)
9	L (3)	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	1	Chemie (PEF)

Sequence modifications

2: 19 - 872 (UniProt: Q14416)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Pro 18	-	expression tag
Leu 873	-	expression tag
Glu 874	-	expression tag
Val 875	-	expression tag
Leu 876	-	expression tag
Phe 877	-	expression tag
Gln 878	-	expression tag

3: 23 - 879 (UniProt: Q14832)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Ala 1	-	expression tag
Pro 2	-	expression tag
Trp 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Pro 6	-	expression tag
Gln 7	-	expression tag
Phe 8	-	expression tag
Glu 9	-	expression tag
Lys 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
Trp 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Pro 18	-	expression tag
Gln 19	-	expression tag
Phe 20	-	expression tag
Glu 21	-	expression tag
Lys 22	-	expression tag
Leu 880	-	expression tag
Glu 881	-	expression tag
Val 882	-	expression tag
Leu 883	-	expression tag
Phe 884	-	expression tag
Gln 885	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	conflict
Ala 203	Gly 203	conflict
Ala 245	Glu 245	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	283514.2
Non-Polymers*	Number of molecules	7
	Total formula weight	2491.1
All*	Total formula weight	286005.2

*Water molecules are not included.