8J9W

Cryo-EM structure of the African swine fever virus topoisomerase 2 complexed with Cut02bDNA and etoposide (EDI-2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA topoisomerase 2	polymer	1197	136422.6	2	UniProt (A0A0A1E3Q0) Pfam (PF16898) Pfam (PF00521) UniProt (by SIFTS) (Q00942)	African swine fever virus
2	C, D (E, C)	DNA (5'-D(*AP*AP*GP*AP*AP*CP*TP*CP*TP*GP*TP*AP*G)-3')	polymer	13	3999.6	2		Homo sapiens
3	E, F (D, F)	DNA (5'-D(*CP*AP*TP*GP*CP*TP*AP*CP*AP*GP*AP*GP*TP*TP*CP*TP*T)-3')	polymer	17	5177.4	2		Homo sapiens
4	G, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J (D)	(5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydrofuro[3',4':6,7]naphtho[2,3-d][1,3]dioxol -5-yl 4,6-O-[(1R)-ethylidene]-beta-D-glucopyranoside	non-polymer		588.6	2	Chemie (EVP)

Sequence modifications

A, B: 1 - 1192 (UniProt: A0A0A1E3Q0)

PDB	External Database	Details
His 1193	-	expression tag
His 1194	-	expression tag
His 1195	-	expression tag
His 1196	-	expression tag
His 1197	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	291199.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1225.7
All*	Total formula weight	292424.9

*Water molecules are not included.