8J9D

Crystal structure of M61 peptidase (bestatin-bound) from Xanthomonas campestris

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative glycyl aminopeptidase	polymer	647	71959.1	4	UniProt (B0RY21) Pfam (PF17899) Pfam (PF07720) Pfam (PF05299) In PDB	Xanthomonas campestris pv. campestris B100
2	A, B, C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A, B, C, D	2-(3-AMINO-2-HYDROXY-4-PHENYL-BUTYRYLAMINO)-4-METHYL-PENTANOIC ACID	non-polymer		308.4	4	Chemie (BES)
4	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	C, D	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
6		water	water		18.0	2172	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 626 (UniProt: B0RY21)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ala 22	Gln 22	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	287836.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1831.5
All*	Total formula weight	289668.0

*Water molecules are not included.