8J6Q
Cryo-EM structure of the 3-HB and compound 9n-bound human HCAR2-Gi1 complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 352 | 40225.8 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | S | single Fab chain (scFv16) | polymer | 250 | 26610.6 | 1 | synthetic construct | ||
5 | R | Hydroxycarboxylic acid receptor 2 | polymer | 295 | 34366.1 | 1 | UniProt (Q8TDS4) Pfam (PF00001) In PDB | Homo sapiens (human) | G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor |
6 | R | 7-methyl-N-[(2R)-1-phenoxypropan-2-yl]-3-(4-propan-2-ylphenyl)pyrazolo[1,5-a]pyrimidine-6-carboxamide | non-polymer | 428.5 | 1 | Chemie (IX8) | |||
7 | R | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
8 | R | (3R)-3-hydroxybutanoic acid | non-polymer | 104.1 | 1 | Chemie (3HR) | |||
9 | R | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
10 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 3 - 354 (UniProt: P63096)
R: 8 - 301 (UniProt: Q8TDS4)
PDB | External Database | Details |
---|---|---|
Asn 47 | Ser 47 | conflict |
Ala 203 | Gly 203 | conflict |
Ala 245 | Glu 245 | conflict |
Ser 326 | Ala 326 | conflict |
PDB | External Database | Details |
---|---|---|
Asn 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 144863.6 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 1527.1 | |
All* | Total formula weight | 146390.7 |