8J6Q

Cryo-EM structure of the 3-HB and compound 9n-bound human HCAR2-Gi1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	352	40225.8	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	single Fab chain (scFv16)	polymer	250	26610.6	1		synthetic construct
5	E (R)	Hydroxycarboxylic acid receptor 2	polymer	295	34366.1	1	UniProt (Q8TDS4) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor
6	F (R)	7-methyl-N-[(2R)-1-phenoxypropan-2-yl]-3-(4-propan-2-ylphenyl)pyrazolo[1,5-a]pyrimidine-6-carboxamide	non-polymer		428.5	1	Chemie (IX8)
7	G, I (R)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
8	H (R)	(3R)-3-hydroxybutanoic acid	non-polymer		104.1	1	Chemie (3HR)
9	J (R)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
10	K (R)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 3 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	conflict
Ala 203	Gly 203	conflict
Ala 245	Glu 245	conflict
Ser 326	Ala 326	conflict

R: 8 - 301 (UniProt: Q8TDS4)

PDB	External Database	Details
Asn 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	144863.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1527.1
All*	Total formula weight	146390.7

*Water molecules are not included.