8J12
Cryo-EM structure of the AsCas12f-sgRNA-target DNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Transposase IS605 OrfB C-terminal domain-containing protein | polymer | 432 | 49925.0 | 2 | UniProt (A0A2U3D0N8) Pfam (PF07282) | Acidibacillus sulfuroxidans | AsCas12f protein |
| 2 | C (D) | DNA (38-MER) | polymer | 38 | 11694.6 | 1 | Acidibacillus sulfooxidans | ||
| 3 | D (E) | DNA (38-MER) | polymer | 38 | 11689.5 | 1 | Acidibacillus sulfooxidans | ||
| 4 | E (C) | RNA (247-MER) | polymer | 224 | 72329.9 | 1 | Acidibacillus sulfooxidans | ||
| 5 | F, H, J, K, L... (A, B, C) | MAGNESIUM ION | non-polymer | 24.3 | 7 | Chemie (MG) | |||
| 6 | G, I (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 422 (UniProt: A0A2U3D0N8)
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | initiating methionine |
| Gly -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 195564.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 301.0 | |
| All* | Total formula weight | 195865.0 |
