8IZF

Cryo-EM structure of the Lac1-Lip1 (Lip1-S74F) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ceramide synthase LAC1	polymer	418	49049.8	2	UniProt (P28496) Pfam (PF03798)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (baker's yeast)	Very-long-chain ceramide synthase LAC1
2	B, D (B, D)	Ceramide synthase subunit LIP1	polymer	171	19560.1	2	UniProt (Q03579)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (baker's yeast)	LAG1/LAC1-interacting protein 1
3	E, F (B, D)	(4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE	non-polymer		763.1	2	Chemie (6PL)

Sequence modifications

B, D: 1 - 150 (UniProt: Q03579)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Phe 74	Ser 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	137219.9
Non-Polymers*	Number of molecules	2
	Total formula weight	1526.2
All*	Total formula weight	138746.1

*Water molecules are not included.