8IZB

Lysophosphatidylserine receptor GPR174-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	246	29048.9	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	376	40845.6	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody35	polymer	138	15140.7	1		synthetic construct
5	E (R)	Probable G-protein coupled receptor 174	polymer	568	63908.2	1	UniProt (Q9BXC1) Pfam (PF00001)	Homo sapiens (human)
6	F (R)	[(2R)-2-oxidanyl-3-phosphonooxy-propyl] (Z)-octadec-9-enoate	non-polymer		436.5	1	Chemie (UBL)
7	G (R)	SERINE	non-polymer		105.1	1	Chemie (SER)
8	H, I (R)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

A: 1 - 66 (UniProt: P63092)

PDB	External Database	Details
Met 25	Lys 25	engineered mutation
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation
Tyr 63	Leu 63	engineered mutation

A: 205 - 394 (UniProt: P63092)

PDB	External Database	Details
Ala 226	Gly 226	engineered mutation
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Asp 272	Leu 272	engineered mutation
Ser 366	Ala 366	engineered mutation
Ala 372	Ile 372	engineered mutation
Ile 375	Val 375	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 1 - 309 (UniProt: Q9BXC1)

PDB	External Database	Details
Asp -84	-	expression tag
Tyr -83	-	expression tag
Lys -82	-	expression tag
Asp -81	-	expression tag
Asp -80	-	expression tag
Asp -79	-	expression tag
Asp -78	-	expression tag
His -77	-	expression tag
His -76	-	expression tag
His -75	-	expression tag
His -74	-	expression tag
His -73	-	expression tag
His -72	-	expression tag
His -71	-	expression tag
His -70	-	expression tag
Gly -69	-	expression tag
Gln -68	-	expression tag
Pro -67	-	expression tag
Gly -66	-	expression tag
Asn -65	-	expression tag
Gly -64	-	expression tag
Ser -63	-	expression tag
Ala -62	-	expression tag
Phe -61	-	expression tag
Leu -60	-	expression tag
Leu -59	-	expression tag
Ala -58	-	expression tag
Pro -57	-	expression tag
Asn -56	-	expression tag
Gly -55	-	expression tag
Ser -54	-	expression tag
His -53	-	expression tag
Ala -52	-	expression tag
Pro -51	-	expression tag
Asp -50	-	expression tag
His -49	-	expression tag
Asn -48	-	expression tag
Val -47	-	expression tag
Thr -46	-	expression tag
Gln -45	-	expression tag
Gln -44	-	expression tag
Arg -43	-	expression tag
Asp -42	-	expression tag
Glu -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
Gly -37	-	expression tag
Gln -36	-	expression tag
Pro -35	-	expression tag
Gly -34	-	expression tag
Asn -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ala -30	-	expression tag
Phe -29	-	expression tag
Leu -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Pro -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Ala -20	-	expression tag
Pro -19	-	expression tag
Asp -18	-	expression tag
His -17	-	expression tag
Asn -16	-	expression tag
Val -15	-	expression tag
Thr -14	-	expression tag
Gln -13	-	expression tag
Gln -12	-	expression tag
Arg -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag

R: 310 - 483 (PDB: 8IZB)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	156804.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1314.9
All*	Total formula weight	158119.5

*Water molecules are not included.