8IZB

Lysophosphatidylserine receptor GPR174-Gs complex

Summary for 8IZB

• Entry DOI: 10.2210/pdb8izb/pdb
• EMDB information: 35838
• Descriptor: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (8 entities in total)
• Functional Keywords: complex, signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 158119.48

Authors

Gong, W.,Liu, G.,Li, X.,Zhang, X. (deposition date: 2023-04-06, release date: 2023-11-01, Last modification date: 2024-10-30)

Primary citation

Liu, G.,Li, X.,Wang, Y.,Zhang, X.,Gong, W.
Structural basis for ligand recognition and signaling of the lysophosphatidylserine receptors GPR34 and GPR174.
Plos Biol., 21:e3002387-e3002387, 2023

PubMed Abstract: Lysophosphatidylserine (LysoPS) is a naturally occurring lipid mediator involved in various physiological and pathological processes especially those related to the immune system. GPR34, GPR174, and P2Y10 have been identified as the receptors for LysoPS, and its analogues have been developed as agonists or antagonists for these receptors. However, the lack of structural information hinders the drug development with novel characteristics, such as nonlipid ligands and allosteric modulators. Here, we determined the structures of human GPR34 and GPR174 in complex with LysoPS and G protein by cryo-EM. Combined with structural analysis and functional studies, we elucidated the lipid-binding modes of these receptors. By structural comparison, we identified the structural features of GPR34 and GPR174 in active state. Taken together, our findings provide insights into ligand recognition and signaling of LysoPS receptors and will facilitate the development of novel therapeutics for related inflammatory diseases and autoimmune diseases.

PubMed: 38048360
DOI: 10.1371/journal.pbio.3002387

Experimental method

ELECTRON MICROSCOPY (3.06 Å)