8IVY

Beta-Glucosidase BglA mutant E166Q in complex with glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-glucosidase	polymer	479	55078.0	2	UniProt (A0A921BS20) Pfam (PF00232) UniProt (by SIFTS) (P26208)	Acetivibrio thermocellus	BglA
2	C, E (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	D, F (A, B)	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
4	G, H (A, B)	water	water		18.0	992	Chemie (HOH)

Sequence modifications

A, B: 1 - 448 (UniProt: A0A921BS20)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Gln 166	Glu 189	engineered mutation
Leu 449	-	expression tag
Glu 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	110156.0
Non-Polymers*	Number of molecules	4
	Total formula weight	544.5
All*	Total formula weight	110700.5

*Water molecules are not included.