8IP0
Cryo-EM structure of type I-B Cascade bound to a PAM-containing dsDNA target at 3.6 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (J) | Type I-MYXAN CRISPR-associated protein Cmx8 | polymer | 551 | 63703.3 | 1 | UniProt (A0A068N831) | Synechocystis sp. PCC 6714 (Aphanocapsa sp. (strain PCC 6714)) | CRISPR-associated protein Cas8b |
| 2 | B (A) | Type I-MYXAN CRISPR-associated protein Cas5/Cmx5/DevS | polymer | 237 | 26592.4 | 1 | UniProt (A0A068N1Y0) | Synechocystis sp. PCC 6714 (Aphanocapsa sp. (strain PCC 6714)) | CRISPR-associated protein Cas5b, Type I-B CRISPR-associated protein Cas5 |
| 3 | C, D, E, F, G... (B, C, D, N, O...) | Fruiting body developmental protein R-like protein | polymer | 301 | 33789.1 | 7 | UniProt (A0A068N458) | Synechocystis sp. PCC 6714 (Aphanocapsa sp. (strain PCC 6714)) | CRISPR-associated protein Cas7b, Type I-B CRISPR-associated protein Cas8 |
| 4 | H (E) | DNA (5'-D(P*TP*CP*CP*AP*TP*GP*TP*TP*TP*AP*TP*CP*AP*CP*C)-3') | polymer | 15 | 4494.9 | 1 | synthetic construct | ||
| 5 | I (F) | RNA (44-MER) | polymer | 44 | 13975.2 | 1 | synthetic construct | ||
| 6 | J (G) | DNA (41-MER) | polymer | 41 | 12725.2 | 1 | synthetic construct | ||
| 7 | K (P) | DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') | polymer | 10 | 3087.1 | 1 | synthetic construct | ||
| 8 | N, O, P (K, L, M) | CRISPR associated protein Cas11b | polymer | 124 | 14538.5 | 3 | UniProt (A0A068N831) | Synechocystis sp. PCC 6714 (Aphanocapsa sp. (strain PCC 6714)) |
Sequence modifications
J: 33 - 551 (UniProt: A0A068N831)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Pro 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Thr 4 | - | expression tag |
| Gln 5 | - | expression tag |
| Ala 6 | - | expression tag |
| Glu 7 | - | expression tag |
| Ile 8 | - | expression tag |
| Leu 9 | - | expression tag |
| Thr 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Asp 12 | - | expression tag |
| Phe 13 | - | expression tag |
| Asn 14 | - | expression tag |
| Leu 15 | - | expression tag |
| Ala 16 | - | expression tag |
| Glu 17 | - | expression tag |
| Leu 18 | - | expression tag |
| Pro 19 | - | expression tag |
| Ser 20 | - | expression tag |
| Ala 21 | - | expression tag |
| Gln 22 | - | expression tag |
| His 23 | - | expression tag |
| Arg 24 | - | expression tag |
| Ala 25 | - | expression tag |
| Gly 26 | - | expression tag |
| Leu 27 | - | expression tag |
| Ala 28 | - | expression tag |
| Gly 29 | - | expression tag |
| Leu 30 | - | expression tag |
| Ile 31 | - | expression tag |
| Leu 32 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 404717.3 | |
| All* | Total formula weight | 404717.3 |
