8IOD
Cryo-EM structure of the PG-901-bound human melanocortin receptor 5 (MC5R)-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41879.5 | 1 | UniProt (P63096) UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT | polymer | 371 | 40227.0 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (cattle) | G gamma-I |
| 4 | L | PG-901 | polymer | 9 | 1037.2 | 1 | synthetic construct | ||
| 5 | N | Nanobody-35 | polymer | 160 | 17352.5 | 1 | Homo sapiens (human) | ||
| 6 | R | HA signal peptide,Melanocortin receptor 5,LgBiT subunit | polymer | 513 | 56920.5 | 1 | UniProt (P03435) UniProt (P33032) Pfam (PF00001) In PDB | Influenza A virus (strain A/Victoria/3/1975 H3N2) | MC5-R,MC-2 |
| 7 | R | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 18 (UniProt: P63096)
A: 19 - 59 (UniProt: P63092)
A: 60 - 180 (UniProt: P63096)
A: 181 - 361 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
B: 356 - 366 (PDB: 8IOD)
R: -14 - 1 (UniProt: P03435)
R: 2 - 325 (UniProt: P33032)
R: 341 - 498 (PDB: 8IOD)
A: 19 - 59 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Asp 42 | Gly 49 | engineered mutation |
| Asn 43 | Glu 50 | engineered mutation |
| Tyr 56 | Leu 63 | engineered mutation |
A: 181 - 361 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 226 | engineered mutation |
| Asp 226 | Ala 249 | engineered mutation |
| Asp 229 | Ser 252 | engineered mutation |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Asp 239 | Leu 272 | engineered mutation |
| Ser 333 | Ala 366 | engineered mutation |
| Ala 339 | Ile 372 | engineered mutation |
| Ile 342 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | linker |
| Ser 342 | - | linker |
| Ser 343 | - | linker |
| Gly 344 | - | linker |
| Gly 345 | - | linker |
| Gly 346 | - | linker |
| Gly 347 | - | linker |
| Ser 348 | - | linker |
| Gly 349 | - | linker |
| Gly 350 | - | linker |
| Gly 351 | - | linker |
| Gly 352 | - | linker |
| Ser 353 | - | linker |
| Ser 354 | - | linker |
| Gly 355 | - | linker |
R: -14 - 1 (UniProt: P03435)
R: 2 - 325 (UniProt: P33032)
| PDB | External Database | Details |
|---|---|---|
| Gly 326 | - | linker |
| Ser 327 | - | linker |
| Ser 328 | - | linker |
| Gly 329 | - | linker |
| Gly 330 | - | linker |
| Gly 331 | - | linker |
| Gly 332 | - | linker |
| Ser 333 | - | linker |
| Gly 334 | - | linker |
| Gly 335 | - | linker |
| Gly 336 | - | linker |
| Gly 337 | - | linker |
| Ser 338 | - | linker |
| Ser 339 | - | linker |
| Gly 340 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 165277.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 165317.9 |
