8IN8

Cryo-EM structure of the target ssDNA-bound SIR2-APAZ/Ago-gRNA quaternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Sir2 superfamily protein	polymer	587	66645.9	1	UniProt (Q74DF6) Pfam (PF13289) In PDB	Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
2	C	Piwi domain protein	polymer	473	53325.6	1	UniProt (Q74DF5) In PDB	Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
3	I	RNA (5'-R(P*AP*UP*AP*AP*UP*GP*GP*UP*UP*UP*CP*UP*UP*AP*GP*AP*CP*GP*UP*CP*GP*UP*UP*U)-3')	polymer	24	7610.5	1		Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
4	J	DNA (5'-D(P*AP*AP*CP*GP*AP*CP*GP*TP*CP*TP*AP*AP*GP*AP*AP*AP*CP*CP*AP*TP*TP*AP*A)-3')	polymer	23	7050.6	1		Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
5	I	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134632.6
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	134656.9

*Water molecules are not included.