8IHY
X-ray crystal structure of Q387E mutant of endo-1,4-beta glucanase from Eisenia fetida
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Endoglucanase | polymer | 462 | 51706.1 | 1 | UniProt (I2FI81) | Eisenia fetida (common brandling worm) | |
| 2 | B (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 3 | C (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | D (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | E (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | F (A) | water | water | 18.0 | 421 | Chemie (HOH) |
Sequence modifications
A: 22 - 456 (UniProt: I2FI81)
| PDB | External Database | Details |
|---|---|---|
| Glu 18 | - | expression tag |
| Ala 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Phe 21 | - | expression tag |
| Glu 387 | Gln 387 | engineered mutation |
| Phe 457 | - | expression tag |
| Leu 458 | - | expression tag |
| Glu 459 | - | expression tag |
| Gln 460 | - | expression tag |
| Lys 461 | - | expression tag |
| Leu 462 | - | expression tag |
| Ile 463 | - | expression tag |
| Ser 464 | - | expression tag |
| Glu 465 | - | expression tag |
| Glu 466 | - | expression tag |
| Asp 467 | - | expression tag |
| Leu 468 | - | expression tag |
| Asn 469 | - | expression tag |
| Ser 470 | - | expression tag |
| Ala 471 | - | expression tag |
| Val 472 | - | expression tag |
| Asp 473 | - | expression tag |
| His 474 | - | expression tag |
| His 475 | - | expression tag |
| His 476 | - | expression tag |
| His 477 | - | expression tag |
| His 478 | - | expression tag |
| His 479 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 51706.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 179.5 | |
| All* | Total formula weight | 51885.5 |
