8IHY

X-ray crystal structure of Q387E mutant of endo-1,4-beta glucanase from Eisenia fetida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	53.360, 71.639, 55.184
Unit cell angles	90.00, 114.04, 90.00

Refinement procedure

Resolution	40.290 - 1.600
R-factor	0.1486
Rwork	0.147
R-free	0.17670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.799
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmeas	0.112	0.523
Number of reflections	49767	2443
<I/σ(I)>	8.3	2.5
Completeness [%]	99.4
Redundancy	3.5
CC(1/2)	0.996	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% PEG3350, 0.2 M magnesium chloride, 50 mM sodium acetate pH 5.0