8IHK
Cryo-EM structure of HCA3-Gi complex with acifran (local)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Soluble cytochrome b562,Hydroxycarboxylic acid receptor 3 | polymer | 530 | 60749.4 | 1 | UniProt (P0ABE7) UniProt (P49019) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,G-protein coupled receptor 109B,G-protein coupled receptor HM74,G-protein coupled receptor HM74B,Niacin receptor 2,Nicotinic acid receptor 2 |
| 2 | B (R) | (5~{S})-5-methyl-4-oxidanylidene-5-phenyl-furan-2-carboxylic acid | non-polymer | 218.2 | 1 | Chemie (P9X) |
Sequence modifications
R: -104 - 0 (UniProt: P0ABE7)
R: 2 - 387 (UniProt: P49019)
| PDB | External Database | Details |
|---|---|---|
| Met -128 | - | initiating methionine |
| Lys -127 | - | expression tag |
| Thr -126 | - | expression tag |
| Ile -125 | - | expression tag |
| Ile -124 | - | expression tag |
| Ala -123 | - | expression tag |
| Leu -122 | - | expression tag |
| Ser -121 | - | expression tag |
| Tyr -120 | - | expression tag |
| Ile -119 | - | expression tag |
| Phe -118 | - | expression tag |
| Cys -117 | - | expression tag |
| Leu -116 | - | expression tag |
| Val -115 | - | expression tag |
| Phe -114 | - | expression tag |
| Ala -113 | - | expression tag |
| Asp -112 | - | expression tag |
| Tyr -111 | - | expression tag |
| Lys -110 | - | expression tag |
| Asp -109 | - | expression tag |
| Asp -108 | - | expression tag |
| Asp -107 | - | expression tag |
| Asp -106 | - | expression tag |
| Lys -105 | - | expression tag |
| Trp -98 | Met 29 | engineered mutation |
| Ile -3 | His 124 | engineered mutation |
| Leu 1 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Glu 388 | - | expression tag |
| Asn 389 | - | expression tag |
| Leu 390 | - | expression tag |
| Tyr 391 | - | expression tag |
| Phe 392 | - | expression tag |
| Gln 393 | - | expression tag |
| Gly 394 | - | expression tag |
| Ser 395 | - | expression tag |
| His 396 | - | expression tag |
| His 397 | - | expression tag |
| His 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
| His 401 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 60749.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 218.2 | |
| All* | Total formula weight | 60967.6 |
