8IHK

Cryo-EM structure of HCA3-Gi complex with acifran (local)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Soluble cytochrome b562,Hydroxycarboxylic acid receptor 3	polymer	530	60749.4	1	UniProt (P0ABE7) UniProt (P49019) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,G-protein coupled receptor 109B,G-protein coupled receptor HM74,G-protein coupled receptor HM74B,Niacin receptor 2,Nicotinic acid receptor 2
2	R	(5~{S})-5-methyl-4-oxidanylidene-5-phenyl-furan-2-carboxylic acid	non-polymer		218.2	1	Chemie (P9X)

Sequence modifications

R: -104 - 0 (UniProt: P0ABE7)

PDB	External Database	Details
Met -128	-	initiating methionine
Lys -127	-	expression tag
Thr -126	-	expression tag
Ile -125	-	expression tag
Ile -124	-	expression tag
Ala -123	-	expression tag
Leu -122	-	expression tag
Ser -121	-	expression tag
Tyr -120	-	expression tag
Ile -119	-	expression tag
Phe -118	-	expression tag
Cys -117	-	expression tag
Leu -116	-	expression tag
Val -115	-	expression tag
Phe -114	-	expression tag
Ala -113	-	expression tag
Asp -112	-	expression tag
Tyr -111	-	expression tag
Lys -110	-	expression tag
Asp -109	-	expression tag
Asp -108	-	expression tag
Asp -107	-	expression tag
Asp -106	-	expression tag
Lys -105	-	expression tag
Trp -98	Met 29	engineered mutation
Ile -3	His 124	engineered mutation
Leu 1	-	linker

R: 2 - 387 (UniProt: P49019)

PDB	External Database	Details
Glu 388	-	expression tag
Asn 389	-	expression tag
Leu 390	-	expression tag
Tyr 391	-	expression tag
Phe 392	-	expression tag
Gln 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	60749.4
Non-Polymers*	Number of molecules	1
	Total formula weight	218.2
All*	Total formula weight	60967.6

*Water molecules are not included.