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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

8IHF

Cryo-EM structure of HCA2-Gi complex with MK6892

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1AGuanine nucleotide-binding protein G(i) subunit alpha-1polymer35440446.01UniProt (P63096)
Pfam (PF00503)
In PDB		Homo sapiens (human)Adenylate cyclase-inhibiting G alpha protein
2SscFv16polymer24826466.51synthetic construct
3BGuanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1polymer38241772.61UniProt (P62874)
Pfam (PF00400)
In PDB		Mus musculus (house mouse)
4CGuanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2polymer707729.91UniProt (P63213)
Pfam (PF00631)
In PDB		Mus musculus (house mouse)G gamma-I
5RSoluble cytochrome b562,Hydroxycarboxylic acid receptor 2polymer65874879.71UniProt (P0ABE7)
UniProt (Q8TDS4)
Pfam (PF07361)
Pfam (PF00001)
In PDB		Escherichia coliCytochrome b-562,G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor
6R2-acetamido-2-deoxy-beta-D-glucopyranosenon-polymer221.21Chemie (NAG)
7R2-[[2,2-dimethyl-3-[3-(5-oxidanylpyridin-2-yl)-1,2,4-oxadiazol-5-yl]propanoyl]amino]cyclohexene-1-carboxylic acidnon-polymer386.41Chemie (FI7)
Sequence modifications
A: 1 - 354 (UniProt: P63096)
PDBExternal DatabaseDetails
Ala 203Gly 203engineered mutation
Ala 219Thr 219conflict
Gln 288Pro 288conflict
Ser 326Ala 326engineered mutation
B: 2 - 340 (UniProt: P62874)
PDBExternal DatabaseDetails
Met -15-initiating methionine
His -14-expression tag
His -13-expression tag
His -12-expression tag
His -11-expression tag
His -10-expression tag
His -9-expression tag
His -8-expression tag
His -7-expression tag
Glu -6-expression tag
Asn -5-expression tag
Leu -4-expression tag
Tyr -3-expression tag
Phe -2-expression tag
Gln -1-expression tag
Gly 0-expression tag
Ser 1-expression tag
Gly 341-expression tag
Gly 342-expression tag
Ser 343-expression tag
Gly 344-expression tag
Gly 345-expression tag
Gly 346-expression tag
Gly 347-expression tag
Ser 348-expression tag
Gly 349-expression tag
Gly 350-expression tag
Ser 351-expression tag
Ser 352-expression tag
Ser 353-expression tag
Gly 354-expression tag
Gly 355-expression tag
Val 356-expression tag
Ser 357-expression tag
Gly 358-expression tag
Trp 359-expression tag
Arg 360-expression tag
Leu 361-expression tag
Phe 362-expression tag
Lys 363-expression tag
Lys 364-expression tag
Ile 365-expression tag
Ser 366-expression tag
R: -104 - 0 (UniProt: P0ABE7)
PDBExternal DatabaseDetails
Met -128-initiating methionine
Lys -127-expression tag
Thr -126-expression tag
Ile -125-expression tag
Ile -124-expression tag
Ala -123-expression tag
Leu -122-expression tag
Ser -121-expression tag
Tyr -120-expression tag
Ile -119-expression tag
Phe -118-expression tag
Cys -117-expression tag
Leu -116-expression tag
Val -115-expression tag
Phe -114-expression tag
Ala -113-expression tag
Asp -112-expression tag
Tyr -111-expression tag
Lys -110-expression tag
Asp -109-expression tag
Asp -108-expression tag
Asp -107-expression tag
Asp -106-expression tag
Lys -105-expression tag
Trp -98Met 29engineered mutation
Ile -3His 124engineered mutation
Leu 1-linker
R: 2 - 363 (UniProt: Q8TDS4)
PDBExternal DatabaseDetails
Glu 364-expression tag
Asn 365-expression tag
Leu 366-expression tag
Tyr 367-expression tag
Phe 368-expression tag
Gln 369-expression tag
Gly 370-expression tag
Ser 371-expression tag
Val 372-expression tag
Phe 373-expression tag
Thr 374-expression tag
Leu 375-expression tag
Glu 376-expression tag
Asp 377-expression tag
Phe 378-expression tag
Val 379-expression tag
Gly 380-expression tag
Asp 381-expression tag
Trp 382-expression tag
Glu 383-expression tag
Gln 384-expression tag
Thr 385-expression tag
Ala 386-expression tag
Ala 387-expression tag
Tyr 388-expression tag
Asn 389-expression tag
Leu 390-expression tag
Asp 391-expression tag
Gln 392-expression tag
Val 393-expression tag
Leu 394-expression tag
Glu 395-expression tag
Gln 396-expression tag
Gly 397-expression tag
Gly 398-expression tag
Val 399-expression tag
Ser 400-expression tag
Ser 401-expression tag
Leu 402-expression tag
Leu 403-expression tag
Gln 404-expression tag
Asn 405-expression tag
Leu 406-expression tag
Ala 407-expression tag
Val 408-expression tag
Ser 409-expression tag
Val 410-expression tag
Thr 411-expression tag
Pro 412-expression tag
Ile 413-expression tag
Gln 414-expression tag
Arg 415-expression tag
Ile 416-expression tag
Val 417-expression tag
Arg 418-expression tag
Ser 419-expression tag
Gly 420-expression tag
Glu 421-expression tag
Asn 422-expression tag
Ala 423-expression tag
Leu 424-expression tag
Lys 425-expression tag
Ile 426-expression tag
Asp 427-expression tag
Ile 428-expression tag
His 429-expression tag
Val 430-expression tag
Ile 431-expression tag
Ile 432-expression tag
Pro 433-expression tag
Tyr 434-expression tag
Glu 435-expression tag
Gly 436-expression tag
Leu 437-expression tag
Ser 438-expression tag
Ala 439-expression tag
Asp 440-expression tag
Gln 441-expression tag
Met 442-expression tag
Ala 443-expression tag
Gln 444-expression tag
Ile 445-expression tag
Glu 446-expression tag
Glu 447-expression tag
Val 448-expression tag
Phe 449-expression tag
Lys 450-expression tag
Val 451-expression tag
Val 452-expression tag
Tyr 453-expression tag
Pro 454-expression tag
Val 455-expression tag
Asp 456-expression tag
Asp 457-expression tag
His 458-expression tag
His 459-expression tag
Phe 460-expression tag
Lys 461-expression tag
Val 462-expression tag
Ile 463-expression tag
Leu 464-expression tag
Pro 465-expression tag
Tyr 466-expression tag
Gly 467-expression tag
Thr 468-expression tag
Leu 469-expression tag
Val 470-expression tag
Ile 471-expression tag
Asp 472-expression tag
Gly 473-expression tag
Val 474-expression tag
Thr 475-expression tag
Pro 476-expression tag
Asn 477-expression tag
Met 478-expression tag
Leu 479-expression tag
Asn 480-expression tag
Tyr 481-expression tag
Phe 482-expression tag
Gly 483-expression tag
Arg 484-expression tag
Pro 485-expression tag
Tyr 486-expression tag
Glu 487-expression tag
Gly 488-expression tag
Ile 489-expression tag
Ala 490-expression tag
Val 491-expression tag
Phe 492-expression tag
Asp 493-expression tag
Gly 494-expression tag
Lys 495-expression tag
Lys 496-expression tag
Ile 497-expression tag
Thr 498-expression tag
Val 499-expression tag
Thr 500-expression tag
Gly 501-expression tag
Thr 502-expression tag
Leu 503-expression tag
Trp 504-expression tag
Asn 505-expression tag
Gly 506-expression tag
Asn 507-expression tag
Lys 508-expression tag
Ile 509-expression tag
Ile 510-expression tag
Asp 511-expression tag
Glu 512-expression tag
Arg 513-expression tag
Leu 514-expression tag
Ile 515-expression tag
Thr 516-expression tag
Pro 517-expression tag
Asp 518-expression tag
Gly 519-expression tag
Ser 520-expression tag
Met 521-expression tag
Leu 522-expression tag
Phe 523-expression tag
Arg 524-expression tag
Val 525-expression tag
Thr 526-expression tag
Ile 527-expression tag
Asn 528-expression tag
Ser 529-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains5
Total formula weight191294.7
Non-Polymers*Number of molecules2
Total formula weight607.6
All*Total formula weight191902.3
*Water molecules are not included.

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PDB entries from 2024-07-24

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