8IH6

Crystal structure of decarboxylase-hydratase complex from Pseudomonas species AP-3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E, F (A, B, C, D, E)	2-oxopent-4-enoate hydratase	polymer	266	28081.0	5	UniProt (Q9KWS4) Pfam (PF01557)	Pseudomonas sp.	2-keto-4-pentenoate hydratase
2	C, G, H, I, J (F, G, H, I, J)	4-oxalocrotonate decarboxylase	polymer	258	27368.3	5	UniProt (Q9KWS3) Pfam (PF01557)	Pseudomonas sp.
3	K, L, M, N (B, G, H, J)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	O, P, Q, R, S... (A, B, F, C, D...)	water	water		18.0	593	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 261 (UniProt: Q9KWS4)

PDB	External Database	Details
Ala -4	-	expression tag
Asp -3	-	expression tag
Leu -2	-	expression tag
Asn -1	-	expression tag
Trp 0	-	expression tag

F, G, H, I, J: 1 - 256 (UniProt: Q9KWS3)

PDB	External Database	Details
Ala -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	277246.6
Non-Polymers*	Number of molecules	4
	Total formula weight	368.4
All*	Total formula weight	277615.0

*Water molecules are not included.