8IH6
Crystal structure of decarboxylase-hydratase complex from Pseudomonas species AP-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.976, 107.792, 164.847 |
| Unit cell angles | 90.00, 124.46, 90.00 |
Refinement procedure
| Resolution | 30.352 - 2.519 |
| R-factor | 0.1772 |
| Rwork | 0.174 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.352 | 2.580 |
| High resolution limit [Å] | 2.519 | 2.520 |
| Number of reflections | 99493 | 7294 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.992 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | potassium sodium tartrate tetrahydrate,BIS-TRIS,polyethylene glycol 6000 |
