8IFM
Cryo-EM structure of tetrameric SPARTA gRNA-ssDNA target complex in state 2
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, F, J, N | TIR domain-containing protein | polymer | 450 | 53256.7 | 4 | UniProt (A0A1I7NFG5) Pfam (PF13676) In PDB | Thermoflavifilum thermophilum | CrtTIR-APAZ |
2 | B, G, K, O | Piwi domain-containing protein | polymer | 507 | 58304.8 | 4 | UniProt (A0A1I7NFD7) In PDB | Thermoflavifilum thermophilum | CrtAgo |
3 | C, H, L, P | guide RNA | polymer | 21 | 6659.0 | 4 | Escherichia coli 'BL21-Gold(DE3)pLysS AG' | ||
4 | D, I, M, Q | target ssDNA | polymer | 25 | 7681.0 | 4 | Escherichia coli 'BL21-Gold(DE3)pLysS AG' | ||
5 | K, C, H, P | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 16 |
Total formula weight | 503606.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 97.2 | |
All* | Total formula weight | 503703.5 |