8IED

Cryo-EM structure of GPR156-miniGo-scFv16 complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Probable G-protein coupled receptor 156	polymer	598	65628.5	2	UniProt (Q8NFN8) Pfam (PF00003)	Homo sapiens (human)
2	C (C)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	228	25451.2	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
3	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	E (N)	Single-chain variable fragment scFv16	polymer	259	27740.9	1		Mus musculus
5	F (Y)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)
6	G, H (A, B)	[(2R)-3-[(E)-hexadec-9-enoyl]oxy-2-octadecanoyloxy-propyl] 2-(trimethylazaniumyl)ethyl phosphate	non-polymer		760.1	2	Chemie (A1LYA)

Sequence modifications

A, B: 1 - 557 (UniProt: Q8NFN8)

PDB	External Database	Details
Ser 558	-	expression tag
Gly 559	-	expression tag
Ser 560	-	expression tag
Gly 561	-	expression tag
Ser 562	-	expression tag
Gly 563	-	expression tag
Arg 564	-	expression tag
Gly 565	-	expression tag
Arg 566	-	expression tag
Gly 567	-	expression tag
Gly 568	-	expression tag
Ser 569	-	expression tag
Glu 570	-	expression tag
Asn 571	-	expression tag
Leu 572	-	expression tag
Tyr 573	-	expression tag
Phe 574	-	expression tag
Gln 575	-	expression tag
Gly 576	-	expression tag
Gly 577	-	expression tag
Ser 578	-	expression tag
Gly 579	-	expression tag
Ser 580	-	expression tag
Gly 581	-	expression tag
Gly 582	-	expression tag
Asp 583	-	expression tag
Tyr 584	-	expression tag
Lys 585	-	expression tag
Asp 586	-	expression tag
Asp 587	-	expression tag
Asp 588	-	expression tag
Asp 589	-	expression tag
Lys 590	-	expression tag
Asp 591	-	expression tag
Tyr 592	-	expression tag
Lys 593	-	expression tag
Asp 594	-	expression tag
Asp 595	-	expression tag
Asp 596	-	expression tag
Asp 597	-	expression tag
Lys 598	-	expression tag

C: 1 - 56 (PDB: 8IED)

PDB	External Database	Details
His 57	-	linker
Gly 58	-	linker
Gly 59	-	linker
Ser 60	-	linker
Gly 61	-	linker
Gly 62	-	linker
Ser 63	-	linker
Gly 64	-	linker
Gly 65	-	linker

C: 66 - 228 (UniProt: P09471)

PDB	External Database	Details
Asp 111	Ala 227	conflict
Asp 114	Gly 230	conflict
-	Asp 232	deletion
-	Gln 233	deletion
-	Val 234	deletion
-	Leu 235	deletion
-	His 236	deletion
-	Glu 237	deletion
-	Asp 238	deletion
-	Glu 239	deletion
-	Thr 240	deletion
-	Thr 241	deletion
Ala 206	Ile 332	conflict
Ile 209	Val 335	conflict

D: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	231728.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1520.2
All*	Total formula weight	233248.4

*Water molecules are not included.