8IEC
Cryo-EM structure of miniGo-scFv16 of GPR156-miniGo-scFv16 complex (local refine)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 228 | 25451.2 | 1 | UniProt (P09471) Pfam (PF00503) | Homo sapiens (human) | |
| 2 | B (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (N) | Single-chain variable fragment scFv16 | polymer | 261 | 27953.1 | 1 | Mus musculus | ||
| 4 | D (Y) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
Sequence modifications
C: 1 - 56 (UniProt: P09471)
C: 66 - 228 (UniProt: P09471)
D: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 9 | Glu 9 | conflict |
| Lys 10 | Arg 10 | conflict |
| Val 13 | Leu 13 | conflict |
| Met 18 | Ala 18 | conflict |
| Asp 42 | Gly 42 | conflict |
| Asn 43 | Glu 43 | conflict |
| PDB | External Database | Details |
|---|---|---|
| His 57 | - | linker |
| Gly 58 | - | linker |
| Gly 59 | - | linker |
| Ser 60 | - | linker |
| Gly 61 | - | linker |
| Gly 62 | - | linker |
| Ser 63 | - | linker |
| Gly 64 | - | linker |
| Gly 65 | - | linker |
| Asp 111 | Ala 227 | conflict |
| Asp 114 | Gly 230 | conflict |
| - | Asp 232 | deletion |
| - | Gln 233 | deletion |
| - | Val 234 | deletion |
| - | Leu 235 | deletion |
| - | His 236 | deletion |
| - | Glu 237 | deletion |
| - | Asp 238 | deletion |
| - | Glu 239 | deletion |
| - | Thr 240 | deletion |
| - | Thr 241 | deletion |
| Ala 206 | Ile 332 | conflict |
| Ile 209 | Val 335 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 100683.5 | |
| All* | Total formula weight | 100683.5 |
