8IBU

Cryo-EM structure of the erythromycin-bound motilin receptor-Gq protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	361	41724.4	1		Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)
3	E	scFv16	polymer	247	26277.3	1		Homo sapiens (human)
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	R	Soluble cytochrome b562,Motilin receptor,Fusion tag	polymer	741	81667.0	1	UniProt (P0ABE7) UniProt (O43193) Pfam (PF00001) Pfam (PF07361) In PDB	Escherichia coli	Cytochrome b-562,G-protein coupled receptor 38
6	R	ERYTHROMYCIN A	non-polymer		733.9	1	Chemie (ERY)

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

R: -116 - -12 (UniProt: P0ABE7)

PDB	External Database	Details
Met -155	-	initiating methionine
Lys -154	-	expression tag
Thr -153	-	expression tag
Ile -152	-	expression tag
Ile -151	-	expression tag
Ala -150	-	expression tag
Leu -149	-	expression tag
Ser -148	-	expression tag
Tyr -147	-	expression tag
Ile -146	-	expression tag
Phe -145	-	expression tag
Cys -144	-	expression tag
Leu -143	-	expression tag
Val -142	-	expression tag
Phe -141	-	expression tag
Ala -140	-	expression tag
Asp -139	-	expression tag
Tyr -138	-	expression tag
Lys -137	-	expression tag
Asp -136	-	expression tag
Asp -135	-	expression tag
Asp -134	-	expression tag
Asp -133	-	expression tag
Ala -132	-	expression tag
Lys -131	-	expression tag
Leu -130	-	expression tag
Gln -129	-	expression tag
Thr -128	-	expression tag
Met -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
Trp -110	Met 29	conflict
Ile -15	His 124	conflict
Leu -11	-	linker
Ala -10	-	linker
Ser -9	-	linker
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Thr 0	-	linker

R: 1 - 412 (UniProt: O43193)
R: 413 - 585 (PDB: 8IBU)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	197625.6
Non-Polymers*	Number of molecules	1
	Total formula weight	733.9
All*	Total formula weight	198359.5

*Water molecules are not included.