8IAK

Cryo-EM structure of the yeast SPT-ORM2 (ORM2-S3A-N71A) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	chimera of Long chain base biosynthesis protein 1 and Serine palmitoyltransferase 1	polymer	534	59354.7	2	UniProt (Q94IB8) UniProt (P25045) Pfam (PF00155) In PDB	Arabidopsis thaliana (Mouse-ear cress)
2	B, F	Serine palmitoyltransferase 2	polymer	561	63189.7	2	UniProt (P40970) Pfam (PF00155) In PDB	Saccharomyces cerevisiae (baker's yeast)	SPT 2,Long chain base biosynthesis protein 2
3	D, H	Protein ORM2	polymer	216	24787.6	2	UniProt (Q06144) Pfam (PF04061) In PDB	Saccharomyces cerevisiae (baker's yeast)
4	C, G	Serine palmitoyltransferase-regulating protein TSC3	polymer	80	9590.2	2	UniProt (Q3E790) In PDB	Saccharomyces cerevisiae (baker's yeast)	Temperature-sensitive CSG2-mutant suppressor protein 3
5	B, F	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)

Sequence modifications

A, E: 25 - 101 (UniProt: Q94IB8)
A, E: 102 - 558 (UniProt: P25045)
D, H: 1 - 216 (UniProt: Q06144)

PDB	External Database	Details
Ala 46	Ser 46	engineered mutation
Ala 47	Ser 47	engineered mutation
Ala 48	Ser 48	engineered mutation
Ala 71	Asn 71	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	313844.4
Non-Polymers*	Number of molecules	2
	Total formula weight	494.3
All*	Total formula weight	314338.7

*Water molecules are not included.