8I5Z

LDH Mutant P101Q-(An unexpected single-point mutation triggers the unleashing of catalytic potential of a NADH-dependent dehydrogenase)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein	polymer	339	37711.3	4	UniProt (F8A9V0) Pfam (PF00389) Pfam (PF02826)	Thermodesulfatator indicus DSM 15286	lactate dehydrogenase
2	E, F, G, H (A, B, C, D)	water	water		18.0	214	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 325 (UniProt: F8A9V0)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Gln 101	Pro 101	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	150845.2
All*	Total formula weight	150845.2

*Water molecules are not included.