8I5Z

LDH Mutant P101Q-(An unexpected single-point mutation triggers the unleashing of catalytic potential of a NADH-dependent dehydrogenase)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008720	molecular_function	D-lactate dehydrogenase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0051287	molecular_function	NAD binding
B	0008720	molecular_function	D-lactate dehydrogenase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0051287	molecular_function	NAD binding
C	0008720	molecular_function	D-lactate dehydrogenase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0051287	molecular_function	NAD binding
D	0008720	molecular_function	D-lactate dehydrogenase activity
D	0016491	molecular_function	oxidoreductase activity
D	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0051287	molecular_function	NAD binding

Functional Information from PROSITE/UniProt

site_id	PS00065
Number of Residues	28
Details	D_2_HYDROXYACID_DH_1 D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature. AGVIGlGKIGrlvatrlkafgck.VLgYD

Chain	Residue	Details
A	ALA146-ASP173

site_id	PS00670
Number of Residues	23
Details	D_2_HYDROXYACID_DH_2 D-isomer specific 2-hydroxyacid dehydrogenases signature 2. LItqADIIsIHcPltreNfhMfN

Chain	Residue	Details
A	LEU189-ASN211

site_id	PS00671
Number of Residues	17
Details	D_2_HYDROXYACID_DH_3 D-isomer specific 2-hydroxyacid dehydrogenases signature 3. MKpGaILVNtARGgLID

Chain	Residue	Details
A	MET218-ASP234