8I2G

FSHR-Follicle stimulating hormone-compound 716340-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	chimera of Guanine nucleotide-binding protein G(s) subunit alpha and Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	259	30168.2	1	UniProt (Q8R4A8) UniProt (Q5JWF2) Pfam (PF00503)	Cricetulus griseus (Chinese hamster)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nb35	polymer	128	13885.4	1		Lama glama
5	E (R)	Follicle-stimulating hormone receptor	polymer	678	76683.7	1	UniProt (P23945) Pfam (PF01462) Pfam (PF13306) Pfam (PF12369) Pfam (PF00001)	Homo sapiens (human)	FSH-R,Follitropin receptor
6	F (X)	Glycoprotein hormones alpha chain	polymer	92	10183.8	1	UniProt (P01215) Pfam (PF00236)	Homo sapiens (human)	Anterior pituitary glycoprotein hormones common subunit alpha,Choriogonadotropin alpha chain,Chorionic gonadotrophin subunit alpha,CG-alpha,Follicle-stimulating hormone alpha chain,FSH-alpha,Follitropin alpha chain,Luteinizing hormone alpha chain,LSH-alpha,Lutropin alpha chain,Thyroid-stimulating hormone alpha chain,TSH-alpha,Thyrotropin alpha chain
7	G (Y)	Follitropin subunit beta	polymer	109	12241.9	1	UniProt (P01225) Pfam (PF00007)	Homo sapiens (human)	Follicle-stimulating hormone beta subunit,FSH-B,FSH-beta,Follitropin beta chain
8	H (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
9	AA, I, J, K, L... (R)	CHOLESTEROL	non-polymer		386.7	13	Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (304) PubChem (176980071) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (18640088) PubChem (1715243) PubChem (1715244) PubChem (45044259) PubChem (23423981) PubChem (49853509) PubChem (50990013) PubChem (52934537) PubChem (56621683) PubChem (191463) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (368063) PubChem (369319) PubChem (44654663) PubChem (59450329) PubChem (59628412) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (46937002) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (638019) PubChem (59274407) PubChem (1549081) PubChem (1553803) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (89951918) PubChem (90224410) PubChem (5997) PubChem (5283629) PubChem (5284644) PubChem (1551269) PubChem (90070115) PubChem (91298236) PubChem (5315842) PubChem (117965027) PubChem (6432564) PubChem (6710666) PubChem (124146240) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (6419195) PubChem (10861996) PubChem (132837164) PubChem (101624109) PubChem (6994560) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (124225449) PubChem (138967089) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (139566851) PubChem (124429284) PubChem (11463329) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (12120344) PubChem (12156156) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (15939944) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (171828367) PubChem (173516975) PubChem (130459183) PubChem (131992107) PubChem (139568882) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573)
10	N, O, P (R)	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)
11	Q, R (R)	MYRISTIC ACID	non-polymer		228.4	2	Chemie (MYR) PubChem (11005)
12	CA, DA, EA, T (X, Y, R)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
13	BA (R)	N-tert-butyl-N-ethyl-8-methoxy-9-propan-2-yloxy-1-thiophen-2-yl-5,6-dihydroimidazo[5,1-a]isoquinoline-3-carboxamide	non-polymer		467.6	1	Chemie (O6F) PubChem (67530240)

Sequence modifications

A: 6 - 195 (UniProt: Q8R4A8)

PDB	External Database	Details
Met 5	-	initiating methionine
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Thr 204	-	linker

A: 205 - 394 (UniProt: Q5JWF2)

PDB	External Database	Details
Thr 205	Ser 848	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 18 - 695 (UniProt: P23945)

PDB	External Database	Details
Ile 273	Ser 273	engineered mutation

X: 1 - 92 (UniProt: P01215)

PDB	External Database	Details
Leu 17	Phe 41	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	188939.7
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	23
	Total formula weight	7605.0
All*	Total formula weight	196969.0

*Water molecules are not included.