8HVI
Activation mechanism of GPR132 by compound NOX-6-7
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Soluble cytochrome b562,Probable G-protein coupled receptor 132 | polymer | 510 | 57125.3 | 1 | UniProt (P0ABE7) UniProt (Q9UNW8) Pfam (PF07361) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,G2 accumulation protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39489.2 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (H) | scFv16 | polymer | 250 | 26610.6 | 1 | Mus musculus | ||
| 6 | F (R) | 3-methyl-5-[(4-oxidanylidene-4-phenyl-butanoyl)amino]-1-benzofuran-2-carboxylic acid | non-polymer | 351.4 | 1 | Chemie (NFI) |
Sequence modifications
R: -98 - -5 (UniProt: P0ABE7)
R: 1 - 380 (UniProt: Q9UNW8)
B: 1 - 354 (UniProt: P63096)
C: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Met -129 | - | initiating methionine |
| Lys -128 | - | expression tag |
| Thr -127 | - | expression tag |
| Ile -126 | - | expression tag |
| Ile -125 | - | expression tag |
| Ala -124 | - | expression tag |
| Leu -123 | - | expression tag |
| Ser -122 | - | expression tag |
| Tyr -121 | - | expression tag |
| Ile -120 | - | expression tag |
| Phe -119 | - | expression tag |
| Cys -118 | - | expression tag |
| Leu -117 | - | expression tag |
| Val -116 | - | expression tag |
| Phe -115 | - | expression tag |
| Ala -114 | - | expression tag |
| Asp -113 | - | expression tag |
| Tyr -112 | - | expression tag |
| Lys -111 | - | expression tag |
| Asp -110 | - | expression tag |
| Asp -109 | - | expression tag |
| Asp -108 | - | expression tag |
| Asp -107 | - | expression tag |
| Lys -106 | - | expression tag |
| Ala -105 | - | expression tag |
| Asp -104 | - | expression tag |
| Leu -103 | - | expression tag |
| Glu -102 | - | expression tag |
| Asp -101 | - | expression tag |
| Asn -100 | - | expression tag |
| Trp -99 | - | expression tag |
| Ile -4 | - | linker |
| Gln -3 | - | linker |
| Lys -2 | - | linker |
| Tyr -1 | - | linker |
| Leu 0 | - | linker |
B: 1 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 203 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 170045.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 351.4 | |
| All* | Total formula weight | 170396.8 |
