8HUJ
Cryo-EM structure of the J-K-St region of EMCV IRES in complex with eIF4G-HEAT1 and eIF4A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Eukaryotic initiation factor 4A-I | polymer | 428 | 48659.4 | 1 | UniProt (P60842) Pfam (PF00270) Pfam (PF00271) | Homo sapiens (human) | eIF-4A-I,eIF4A-I,ATP-dependent RNA helicase eIF4A-1 |
| 2 | B (B) | Eukaryotic translation initiation factor 4 gamma 1 | polymer | 270 | 31786.8 | 1 | UniProt (Q04637) Pfam (PF02854) | Homo sapiens (human) | eIF-4-gamma 1,eIF-4G 1,eIF-4G1,p220 |
| 3 | C (C) | IRES RNA (J-K-St) | polymer | 108 | 34838.6 | 1 | GenBank (NC_001479.1) | Encephalomyocarditis virus | |
| 4 | D, E, F (C) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | G (C) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 2 - 406 (UniProt: P60842)
B: 746 - 992 (UniProt: Q04637)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | initiating methionine |
| Gly -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 723 | - | initiating methionine |
| Asn 724 | - | expression tag |
| His 725 | - | expression tag |
| Lys 726 | - | expression tag |
| Val 727 | - | expression tag |
| His 728 | - | expression tag |
| His 729 | - | expression tag |
| His 730 | - | expression tag |
| His 731 | - | expression tag |
| His 732 | - | expression tag |
| His 733 | - | expression tag |
| Ile 734 | - | expression tag |
| Glu 735 | - | expression tag |
| Gly 736 | - | expression tag |
| Arg 737 | - | expression tag |
| His 738 | - | expression tag |
| Met 739 | - | expression tag |
| Glu 740 | - | expression tag |
| Leu 741 | - | expression tag |
| Gly 742 | - | expression tag |
| Thr 743 | - | expression tag |
| Leu 744 | - | expression tag |
| Glu 745 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 115284.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 72.9 | |
| All* | Total formula weight | 115357.8 |
