8HRS

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K/P192S) in complex with NADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	342	37149.5	4	UniProt (Q01651) Pfam (PF00044) Pfam (PF02800)	Corynebacterium glutamicum ATCC 13032	GAPDH,NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
2	E, G, I, K (A, B, C, D)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
3	F, H, J (B, C)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	L, M, N, O (A, B, C, D)	water	water		18.0	722	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 334 (UniProt: Q01651)

PDB	External Database	Details
Ser 36	Leu 36	engineered mutation
Lys 37	Thr 37	engineered mutation
Ser 192	Pro 192	engineered mutation
Leu 335	-	expression tag
Glu 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148598.1
Non-Polymers*	Number of molecules	7
	Total formula weight	3249.9
All*	Total formula weight	151848.0

*Water molecules are not included.