8HRS
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K/P192S) in complex with NADP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 88.125, 99.133, 152.878 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 分解能 | 38.970 - 2.000 |
| R因子 | 0.1734 |
| Rwork | 0.171 |
| R-free | 0.22729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8hro |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0403) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.030 |
| 分解能 [Å] (高) | 2.000 | 5.430 | 2.000 |
| Rmerge_l_obs | 0.987 | 0.048 | 0.506 |
| Total number of observations | 669336 | ||
| 独立反射数 | 86317 | 8741 | 8638 |
| <I/σ(I)> | 7.1 | ||
| 完全性 [%] | 99.7 | 99.7 | 99.3 |
| 冗長性 | 3.8 | 4.4 | 3.3 |
| CC(1/2) | 0.994 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, Lithium acetate dihydrate |
