8HRS

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K/P192S) in complex with NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	88.125, 99.133, 152.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.970 - 2.000
R-factor	0.1734
Rwork	0.171
R-free	0.22729
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hro
RMSD bond length	0.009
RMSD bond angle	1.620
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.987	0.048	0.506
Total number of observations	669336
Number of reflections	86317	8741	8638
<I/σ(I)>	7.1
Completeness [%]	99.7	99.7	99.3
Redundancy	3.8	4.4	3.3
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, Lithium acetate dihydrate