8HR5

Cryo-EM structure of the CnCas12f1-sgRNA-DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transposase	polymer	497	58609.7	2	UniProt (A0A386YN77) In PDB	Clostridium novyi	CnCas12f1
2	C	sgRNA	polymer	235	75498.4	1		synthetic construct
3	D	DNA (28-MER)	polymer	28	8612.6	1		synthetic construct
4	E	DNA (5'-D(*TP*AP*AP*CP*CP*TP*AP*AP*TP*AP*GP*AP*TP*GP*TP*GP*AP*A)-3')	polymer	18	5547.6	1		synthetic construct

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	206878.0
All*	Total formula weight	206878.0

*Water molecules are not included.