8HQ2

Crystal structure of human ADAM22 in complex with human LGI1 mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Disintegrin and metalloproteinase domain-containing protein 22	polymer	486	53743.1	2	UniProt (Q9P0K1) Pfam (PF01421) Pfam (PF00200) Pfam (PF08516) Pfam (PF07974)	Homo sapiens (human)	ADAM 22,Metalloproteinase-disintegrin ADAM22-3,Metalloproteinase-like,disintegrin-like,and cysteine-rich protein 2
2	B, D (D, E)	Leucine-rich glioma-inactivated protein 1	polymer	526	60512.4	2	UniProt (O95970) Pfam (PF03736) Pfam (PF13855)	Homo sapiens (human)	Epitempin-1
3	E, F, G, K, O... (A, D, B, E)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
4	H, I, J, L, M... (A, D, B, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)
5	AA, BA, Y, Z (B, E, A, D)	water	water		18.0	174	Chemie (HOH)

Sequence modifications

D, E: 39 - 557 (UniProt: O95970)

PDB	External Database	Details
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
Asn 88	Leu 88	engineered mutation
Ala 89	Phe 89	engineered mutation
Gln 474	Arg 474	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228511.0
Non-Polymers*	Number of molecules	20
	Total formula weight	2975.1
All*	Total formula weight	231486.1

*Water molecules are not included.