8HQ2

Crystal structure of human ADAM22 in complex with human LGI1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97850
Spacegroup name	P 1
Unit cell lengths	53.250, 98.854, 131.258
Unit cell angles	96.21, 100.44, 91.31

Refinement procedure

Resolution	45.830 - 2.930
R-factor	0.247
Rwork	0.245
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w8a 5sxm 3g5c
RMSD bond length	0.004
RMSD bond angle	0.748
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.990
High resolution limit [Å]	2.930	2.930
Rmerge	0.094	0.944
Number of reflections	54802	2716
<I/σ(I)>	21.167	2.125
Completeness [%]	98.4	97.8
Redundancy	6.3	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	7.5% PEG 4000, 0.2M MAGNESIUM CHLORIDE, 0.1M HEPES PH=7.5