8HOY

Cryo-EM structure of monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex without DNA at 2.76 angostram

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	1029	120041.2	1	UniProt (Q5IXW8) Pfam (PF08452) Pfam (PF03104) Pfam (PF08408) Pfam (PF00136) UniProt (by SIFTS) (A0A7H0DN44) In PDB	Monkeypox virus
2	C	DNA polymerase processivity factor component A20	polymer	426	49203.9	1	UniProt (Q5IXP2) Pfam (PF05941) UniProt (by SIFTS) (A0A7H0DNC0) In PDB	Monkeypox virus
3	B	E4R	polymer	241	27883.7	1	UniProt (Q5IXS4) UniProt (by SIFTS) (M1LL92) In PDB	Monkeypox virus

Sequence modifications

A: 1 - 1006 (UniProt: Q5IXW8)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Phe 108	Leu 108	engineered mutation
Leu 411	Trp 411	engineered mutation

B: 1 - 218 (UniProt: Q5IXS4)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	197128.8
All*	Total formula weight	197128.8

*Water molecules are not included.