8HMZ

Cryo-EM structure of the human post-catalytic TSEN/pre-tRNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	tRNA-splicing endonuclease subunit Sen2	polymer	485	55459.0	1	UniProt (Q8NCE0) Pfam (PF02778) Pfam (PF01974) In PDB	Homo sapiens (human)	tRNA-intron endonuclease Sen2,HsSen2
2	B	tRNA-splicing endonuclease subunit Sen34	polymer	330	35827.0	1	UniProt (Q9BSV6) Pfam (PF01974) In PDB	Homo sapiens (human)	Leukocyte receptor cluster member 5,tRNA-intron endonuclease Sen34,HsSen34
3	C	tRNA-splicing endonuclease subunit Sen54	polymer	546	61032.3	1	UniProt (Q7Z6J9) Pfam (PF12928) In PDB	Homo sapiens (human)	SEN54 homolog,HsSEN54,tRNA-intron endonuclease Sen54
4	D	Chromosome 1 open reading frame 19, isoform CRA_a	polymer	213	22796.6	1	UniProt (A0A2U3TZM3) Pfam (PF09631) UniProt (by SIFTS) (Q8WW01) In PDB	Homo sapiens (human)	tRNA-splicing endonuclease subunit Sen15
5	5	Pre-tRNA 5' END	polymer	72	23327.8	1		Homo sapiens
6	3	Pre-tRNA 3' END	polymer	42	13488.0	1		Homo sapiens
7	E	Polyribonucleotide 5'-hydroxyl-kinase Clp1	polymer	445	49837.5	1	UniProt (Q92989) Pfam (PF16573) Pfam (PF16575) Pfam (PF06807) In PDB	Homo sapiens (human)	Polyadenylation factor Clp1,Polynucleotide kinase Clp1,Pre-mRNA cleavage complex II protein Clp1
8	5, 3	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)

Sequence modifications

A: 1 - 465 (UniProt: Q8NCE0)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Ala -8	-	expression tag
Ser -7	-	expression tag
Asp -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Asp -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

B: 1 - 310 (UniProt: Q9BSV6)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Ala -8	-	expression tag
Ser -7	-	expression tag
Asp -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Asp -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

C: 1 - 526 (UniProt: Q7Z6J9)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Ala -8	-	expression tag
Ser -7	-	expression tag
Asp -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Asp -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

D: 1 - 175 (UniProt: A0A2U3TZM3)

PDB	External Database	Details
Met -37	-	initiating methionine
Ala -36	-	expression tag
Ser -35	-	expression tag
Ser -34	-	expression tag
Ala -33	-	expression tag
Trp -32	-	expression tag
Ser -31	-	expression tag
His -30	-	expression tag
Pro -29	-	expression tag
Gln -28	-	expression tag
Phe -27	-	expression tag
Glu -26	-	expression tag
Lys -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Ala -13	-	expression tag
Trp -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
Pro -9	-	expression tag
Gln -8	-	expression tag
Phe -7	-	expression tag
Glu -6	-	expression tag
Lys -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag

E: 1 - 425 (UniProt: Q92989)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Ala -8	-	expression tag
Ser -7	-	expression tag
Asp -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Asp -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	261768.2
Non-Polymers*	Number of molecules	6
	Total formula weight	145.8
All*	Total formula weight	261914.1

*Water molecules are not included.