8HKC
Cryo-EM structure of E. coli RNAP sigma32 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 331 | 36801.0 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1346 | 151341.4 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | F | DNA-directed RNA polymerase subunit beta' | polymer | 1425 | 157613.1 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | G | DNA (54-MER) | polymer | 54 | 16614.6 | 1 | Escherichia coli | ||
| 5 | H | DNA (54-MER) | polymer | 54 | 16663.7 | 1 | Escherichia coli | ||
| 6 | E | RNA polymerase sigma factor RpoH | polymer | 284 | 32513.8 | 1 | UniProt (P0AGB3) Pfam (PF04542) Pfam (PF04545) In PDB | Escherichia coli K-12 | Heat shock regulatory protein F33.4,RNA polymerase sigma-32 factor |
| 7 | F | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 8 | F | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A, B: 1 - 329 (UniProt: P0A7Z4)
C: 2 - 1342 (UniProt: P0A8V2)
F: 2 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Lys 330 | - | expression tag |
| Leu 331 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Glu 0 | - | expression tag |
| Phe 1 | - | expression tag |
| Ser 1343 | - | expression tag |
| Arg 1344 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Thr 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| His 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| His 1414 | - | expression tag |
| His 1415 | - | expression tag |
| His 1416 | - | expression tag |
| His 1417 | - | expression tag |
| His 1418 | - | expression tag |
| His 1419 | - | expression tag |
| His 1420 | - | expression tag |
| His 1421 | - | expression tag |
| Gly 1422 | - | expression tag |
| Thr 1423 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 448348.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 448503.8 |
