Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8HKC

Cryo-EM structure of E. coli RNAP sigma32 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006351	biological_process	DNA-templated transcription
A	0006352	biological_process	DNA-templated transcription initiation
A	0006879	biological_process	intracellular iron ion homeostasis
A	0008023	cellular_component	transcription elongation factor complex
A	0009408	biological_process	response to heat
A	0016020	cellular_component	membrane
A	0016779	molecular_function	nucleotidyltransferase activity
A	0031564	biological_process	transcription antitermination
A	0032784	biological_process	regulation of DNA-templated transcription elongation
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0036460	biological_process	cellular response to cell envelope stress
A	0042128	biological_process	nitrate assimilation
A	0044780	biological_process	bacterial-type flagellum assembly
A	0046983	molecular_function	protein dimerization activity
A	0048870	biological_process	cell motility
A	0071973	biological_process	bacterial-type flagellum-dependent cell motility
A	0090605	biological_process	submerged biofilm formation
A	2000142	biological_process	regulation of DNA-templated transcription initiation
B	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006351	biological_process	DNA-templated transcription
B	0006352	biological_process	DNA-templated transcription initiation
B	0006879	biological_process	intracellular iron ion homeostasis
B	0008023	cellular_component	transcription elongation factor complex
B	0009408	biological_process	response to heat
B	0016020	cellular_component	membrane
B	0016779	molecular_function	nucleotidyltransferase activity
B	0031564	biological_process	transcription antitermination
B	0032784	biological_process	regulation of DNA-templated transcription elongation
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0036460	biological_process	cellular response to cell envelope stress
B	0042128	biological_process	nitrate assimilation
B	0044780	biological_process	bacterial-type flagellum assembly
B	0046983	molecular_function	protein dimerization activity
B	0048870	biological_process	cell motility
B	0071973	biological_process	bacterial-type flagellum-dependent cell motility
B	0090605	biological_process	submerged biofilm formation
B	2000142	biological_process	regulation of DNA-templated transcription initiation
C	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006351	biological_process	DNA-templated transcription
C	0006352	biological_process	DNA-templated transcription initiation
C	0006879	biological_process	intracellular iron ion homeostasis
C	0008023	cellular_component	transcription elongation factor complex
C	0009408	biological_process	response to heat
C	0016020	cellular_component	membrane
C	0016779	molecular_function	nucleotidyltransferase activity
C	0031564	biological_process	transcription antitermination
C	0032549	molecular_function	ribonucleoside binding
C	0032784	biological_process	regulation of DNA-templated transcription elongation
C	0034062	molecular_function	5'-3' RNA polymerase activity
C	0036460	biological_process	cellular response to cell envelope stress
C	0042128	biological_process	nitrate assimilation
C	0044780	biological_process	bacterial-type flagellum assembly
C	0046677	biological_process	response to antibiotic
C	0048870	biological_process	cell motility
C	0071973	biological_process	bacterial-type flagellum-dependent cell motility
C	0090605	biological_process	submerged biofilm formation
C	2000142	biological_process	regulation of DNA-templated transcription initiation
E	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
E	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
E	0001046	molecular_function	core promoter sequence-specific DNA binding
E	0001216	molecular_function	DNA-binding transcription activator activity
E	0003677	molecular_function	DNA binding
E	0003700	molecular_function	DNA-binding transcription factor activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006310	biological_process	DNA recombination
E	0006351	biological_process	DNA-templated transcription
E	0006352	biological_process	DNA-templated transcription initiation
E	0006355	biological_process	regulation of DNA-templated transcription
E	0009009	molecular_function	site-specific recombinase activity
E	0009408	biological_process	response to heat
E	0010468	biological_process	regulation of gene expression
E	0015074	biological_process	DNA integration
E	0016987	molecular_function	sigma factor activity
E	0031421	cellular_component	invertasome
E	0045893	biological_process	positive regulation of DNA-templated transcription
E	2000142	biological_process	regulation of DNA-templated transcription initiation
F	0000287	molecular_function	magnesium ion binding
F	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006351	biological_process	DNA-templated transcription
F	0006352	biological_process	DNA-templated transcription initiation
F	0006879	biological_process	intracellular iron ion homeostasis
F	0008023	cellular_component	transcription elongation factor complex
F	0008270	molecular_function	zinc ion binding
F	0009408	biological_process	response to heat
F	0016020	cellular_component	membrane
F	0016779	molecular_function	nucleotidyltransferase activity
F	0031564	biological_process	transcription antitermination
F	0032784	biological_process	regulation of DNA-templated transcription elongation
F	0034062	molecular_function	5'-3' RNA polymerase activity
F	0036460	biological_process	cellular response to cell envelope stress
F	0042128	biological_process	nitrate assimilation
F	0044780	biological_process	bacterial-type flagellum assembly
F	0046677	biological_process	response to antibiotic
F	0046872	molecular_function	metal ion binding
F	0048870	biological_process	cell motility
F	0071973	biological_process	bacterial-type flagellum-dependent cell motility
F	0090605	biological_process	submerged biofilm formation
F	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnIGLMkAV

Chain	Residue	Details
E	ASP77-VAL90

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TlqELAdrygVSaerVrQLEknaMkkL

Chain	Residue	Details
E	THR252-LEU278

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKMAGrHGNKGV

Chain	Residue	Details
C	GLY1063-VAL1075

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	19
Details	DNA_BIND: H-T-H motif => ECO:0000255\|HAMAP-Rule:MF_00961

Chain	Residue	Details
E	LEU253-LYS272
F	CYS895
F	CYS898
F	CYS72
F	CYS85
F	CYS88
F	ASP460
F	ASP462
F	ASP464
F	CYS814
F	CYS888

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000255\|HAMAP-Rule:MF_01322, ECO:0000269\|PubMed:18723842

Chain	Residue	Details
F	LYS983
B	LYS297

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine; by PatZ => ECO:0000269\|PubMed:21696463

Chain	Residue	Details
A	LYS298
B	LYS298

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)