8HJ7
Crystal structure of barley exohydrolase isoform ExoI E220A mutant in complex with beta-D-glucopyranose.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-D-glucan exohydrolase isoenzyme ExoI | polymer | 604 | 65138.2 | 1 | UniProt (Q9XEI3) Pfam (PF00933) Pfam (PF01915) | Hordeum vulgare | |
| 2 | B, C, D (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 3 | E, F, G, H, I... (A) | ACETATE ION | non-polymer | 59.0 | 7 | Chemie (ACT) | |||
| 4 | L (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | M, N (A) | beta-D-glucopyranose | non-polymer | 180.2 | 2 | Chemie (BGC) | |||
| 6 | O (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 7 | P, Q, R, S, T (A) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 8 | U (A) | water | water | 18.0 | 699 | Chemie (HOH) |
Sequence modifications
A: 0 - 602 (UniProt: Q9XEI3)
| PDB | External Database | Details |
|---|---|---|
| Ala -1 | - | expression tag |
| Ala 220 | Glu 245 | engineered mutation |
| Lys 320 | Asn 345 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 65138.2 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2247.9 | |
| All* | Total formula weight | 67386.1 |
