8HFH

CENP-E motor domain in complex with AMPPNP and Mg2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Centromere-associated protein E	polymer	353	39799.1	1	UniProt (Q02224) Pfam (PF00225)	Homo sapiens (human)	Centromere protein E,CENP-E,Kinesin-7,Kinesin-related protein CENPE
2	B (A)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D, E, F (A)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
5	G (A)	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
6	H (A)	water	water		18.0	302	Chemie (HOH)

Sequence modifications

A: 1 - 339 (UniProt: Q02224)

PDB	External Database	Details
Met -5	-	initiating methionine
Asn -4	-	expression tag
His -3	-	expression tag
Lys -2	-	expression tag
Val -1	-	expression tag
His 0	-	expression tag
Pro 300	Ala 300	engineered mutation
Gly 340	-	expression tag
Ser 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39799.1
Non-Polymers*	Number of molecules	6
	Total formula weight	668.6
All*	Total formula weight	40467.6

*Water molecules are not included.