8HFC

Cryo-EM structure of yeast Erf2/Erf4 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Palmitoyltransferase ERF2	polymer	381	43811.7	1	UniProt (Q06551) Pfam (PF01529) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	DHHC cysteine-rich domain-containing protein ERF2,Ras protein acyltransferase
2	B	Ras modification protein ERF4	polymer	264	29652.7	1	UniProt (P41912) Pfam (PF10256) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Hyperactive Ras suppressor protein 5
3	A	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)
4	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 359 (UniProt: Q06551)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Asp -5	-	expression tag
Glu -4	-	expression tag
Val -3	-	expression tag
Asp -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag

B: 1 - 237 (UniProt: P41912)

PDB	External Database	Details
Met -26	-	initiating methionine
Asp -25	-	expression tag
Tyr -24	-	expression tag
Lys -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Lys -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Lys -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Lys -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Asp -5	-	expression tag
Glu -4	-	expression tag
Val -3	-	expression tag
Asp -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73464.4
Non-Polymers*	Number of molecules	3
	Total formula weight	387.2
All*	Total formula weight	73851.6

*Water molecules are not included.