8HFB
Evolved variant of quercetin 2,4-dioxygenase from Bacillus subtilis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Quercetin 2,3-dioxygenase | polymer | 337 | 37571.5 | 2 | UniProt (P42106) Pfam (PF07883) | Bacillus subtilis | Quercetinase,Flavonol 2,4-dioxygenase |
| 2 | C, D, E, I (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 3 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | G, H, J, K (A, B) | NICKEL (II) ION | non-polymer | 58.7 | 4 | Chemie (NI) | |||
| 5 | L, M (A, B) | water | water | 18.0 | 187 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: P42106)
| PDB | External Database | Details |
|---|---|---|
| Thr 160 | Asp 160 | conflict |
| Leu 221 | Val 221 | engineered mutation |
| Val 231 | Ile 231 | engineered mutation |
| Cys 239 | His 239 | engineered mutation |
| Ala 269 | Val 269 | engineered mutation |
| Phe 286 | Met 286 | engineered mutation |
| Val 290 | Leu 290 | engineered mutation |
| Lys 314 | Glu 314 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 75142.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 575.1 | |
| All* | Total formula weight | 75718.1 |
