8HC1

CryoEM structure of Helicobacter pylori UreFD/urease complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M, Q...	Urease subunit alpha	polymer	238	26587.7	12	UniProt (P14916) Pfam (PF00547) Pfam (PF00699) In PDB	Helicobacter pylori 26695	Urea amidohydrolase subunit alpha
2	B, F, J, N, R...	Urease subunit beta	polymer	569	61759.5	12	UniProt (P69996) Pfam (PF00449) Pfam (PF01979) In PDB	Helicobacter pylori 26695	Urea amidohydrolase subunit beta
3	C, G, K, O, S...	Urease accessory protein UreH	polymer	273	30741.2	12	UniProt (Q09067) Pfam (PF01774) In PDB	Helicobacter pylori 26695	UreD
4	D, H, L, P, T...	Urease accessory protein UreF	polymer	254	28517.6	12	UniProt (Q09065) Pfam (PF01730) In PDB	Helicobacter pylori 26695

Sequence modifications

C, G, K, O, S, W, a, e, i, m, q, u: 1 - 265 (UniProt: Q09067)

PDB	External Database	Details
Ala 140	Glu 140	engineered mutation
Trp 266	-	expression tag
Ser 267	-	expression tag
His 268	-	expression tag
Pro 269	-	expression tag
Gln 270	-	expression tag
Phe 271	-	expression tag
Glu 272	-	expression tag
Lys 273	-	expression tag

D, H, L, P, T, X, b, f, j, n, r, v: 1 - 254 (UniProt: Q09065)

PDB	External Database	Details
Ala 179	Arg 179	engineered mutation
Asp 183	Tyr 183	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	48
	Total formula weight	1771272.6
All*	Total formula weight	1771272.6

*Water molecules are not included.