8H8J

Lodoxamide-bound GPR35 in complex with G13

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein subunit alpha-13	polymer	362	42423.6	1	UniProt (Q14344) Pfam (PF00503) In PDB	Homo sapiens (human)	G alpha-13,G-protein subunit alpha-13
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	G-protein coupled receptor 35	polymer	308	33972.0	1	UniProt (Q9HC97) Pfam (PF00001) In PDB	Homo sapiens (human)	Kynurenic acid receptor,KYNA receptor
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	H	scFv16	polymer	247	26277.3	1		Mus musculus
6	C	2,2'-[(2-chloro-5-cyano-1,3-phenylene)bis(azanediyl)]bis(oxoacetic acid)	non-polymer		311.6	1	Chemie (WYB)
7	C	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
8	C	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)

Sequence modifications

A: 34 - 378 (UniProt: Q14344)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Cys 3	-	expression tag
Thr 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	150761.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1511.7
All*	Total formula weight	152272.8

*Water molecules are not included.